Effect of beta-glucuronidase upon the hydrolysis of corticoids in cat urine.
نویسنده
چکیده
منابع مشابه
Hydrolysis of Conjugates of Urinary Corticoids with /3-glucuronidase
Since urinary steroids are excreted as conjugates, principally of sulfuric and glucuronic acids, hydrolysis is a necessary preliminary procedure which must be carried out before the steroids may be readily extracted by means of organic solvents. The effectiveness of bacterial action in the hydrolysis of conjugates of estrogens was demonstrated by Cohen and Marrian (1) who allowed putrefaction t...
متن کاملHydrolysis of Conjugates of Urinary Corticoids with P-glucuronidase
Hydrolysis of conjugates of urinary neutral reducing lipides by means of /?-glucuronidase of bacterial origin, as described in Paper I of this series (I), permits the extraction from urine of much larger amounts of these substances than may be obtained by extraction of acidified urine in separatory funnels. The isolation of a crystalline reducing steroid from normal urine which had been treated...
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Individuals exposed to carcinogenic aromatic amines excrete arylamine N- and O-glucuronide metabolites. This study assessed the susceptibility of selected glucuronides to hydrolysis by human and Escherichia coli beta-glucuronidase. N- or O-glucuronides were prepared with the following aglycones: benzidine, N-acetylbenzidine, N'-hydroxy-N-acetylbenzidine, N-hydroxy-N-acetylbenzidine, N-hydroxy-N...
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The CEDIA dau Benzodiazepine assay has been reformulated to include online hydrolysis of urinary benzodiazepine glucuronide conjugates. The new antibody possesses enhanced cross-reactivities toward the low-dose benzodiazepines, which are excreted at low urinary drug-metabolite concentrations. The screening method was evaluated using lorazepam as the probe benzodiazepine. Four subjects each cons...
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This study examined the potential of abalone β-glucuronidase as a viable and cost effective alternative to current hydrolysis procedures using acid, Helix pomatia β-glucuronidase and Escherichia coli β-glucuronidase. Abalone β-glucuronidase successfully hydrolyzed oxazepam-glucuronide and lorazepam-glucuronide within 5% of the spiked control concentration. Benzodiazepines present in authentic u...
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عنوان ژورنال:
- The Biochemical journal
دوره 70 4 شماره
صفحات -
تاریخ انتشار 1958